|
KMID : 0380619870190020146
|
|
Korean Journal of Food Science and Technology
1987 Volume.19 No. 2 p.146 ~ p.150
|
|
|
Effects of Salts on the Partition of Proteins in Poly(ethylene glycol) - Dextran Aqueous Two Phase System
|
|
|
|
|
Abstract
|
|
|
|
The effects of pH and added salts on the partition coefficients of proteins in a polyethylene glycol)-dextran aqueous two-phase system were investigated. The partition coefficients attained the lowest value at the isoelectric point of proteins in an equal volume aqueous two-phase system containing 5% PEG and 9.5% dextran in 5 mM phosphate buffer solution. The coefficients increased dramatically at pH 11; BSA which had highest effective hydrophobicity marked 50-fold increase, while β-lactoglobulin and ovalbumin which had low hydrophobicity 10-fold increase, respectively. The effect of added salts varied with the pH. The partition coefficient increased by the addition of salt at pH 3.0 but decreased drastically at pH 7.0. The partition coefficient increased in the order of added Li $lt; Na $lt; K at pH 3.0 and decreased in the order of added Li $gt; Na $gt; K at pH 11.0.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|